| 초록 |
Alzheimer’s disease, the most common neurologic disorder. However, the low clinical significance of the core AD biomarkers remains a challenge. Recently, blood-derived exosomal miRNAs are gaining increasing attention because of their clinical significance in diagnosing of AD. However, quantitative and qualitative detection is difficult due to their characteristics. Here, we present a DNA-assembled advanced plasmonic architecture-based plasmonic biosensor to accurately detect exosomal miRNAs in human serum. The proposed biosensor is capable of selective single-nucleotide detection of exosomal miRNAs at the attomolar level. Furthermore, it identified AD patients from healthy controls by measuring the levels of exosomal miRNA-125b, miRNA-15a, and miRNA-361 in clinical serum samples. These results demonstrate that our sensor has great potential to revolutionize the field of dementia research and treatment in the future. |
