| 초록 |
Amino acids are commercially important as they are used as precursors in industries of foods, pharmaceutical, and cosmetics as well as animal feed additives. We have previously developed 100% rationally engineered E. coli strain capable of 0.38 g L-valine per gram glucose (0.58 mol L-valine per mol glucose) by batch culture. In this study, systems biological strategy of employing flux response analysis in bioprocess development was reported for high-level L-valine production. There existed a trade-off between L-valine production and biomass formation, which was optimized for the most efficient L-valine production. Moreover, acetic acid feeding strategy was optimized based on flux response analysis. The final fed-batch cultivation strategy allowed production of 32.3 g/L L-valine, the highest concentration reported for E. coli. [This work was supported by the Technology Development Program to Solve Climate Changes from the Ministry of Education, Science and Technology (MEST) through the National Research Foundation of Korea (NRF-2012-C1AAA001-2012M1A2A2026556) and by the Advanced Biomass R&D Center of Korea (2011-0028386) through the Global Frontier Research Program of the MEST.] |
