| 초록 |
The endocytosis-mediating performances of two types of peptide ligands, tumor cell receptor binding peptide (TRBP) and tumor cell membrane penetrating peptide(TMPP), were analyzed and compared with a carrier of peptide ligands-human ferritin heavy chain(hFTH) nanoparticle. 24 copies of a TMPP(human immunodeficiency virus-derived TAT peptide) and/or a TRBP (peptide ligand with specific and strong affinity for either human integrin(αvβ3) or epidermal growth factor receptor I(EGFR) that is overexpressed on various cancer cells) were genetically presented on the surface of each hFTH nanopariticle. The quantitative level of intracellular localization and endocytosis of fluorescence dye-labeled TRBP- and TMPP-presenting nanoparticles were estimated in the in vitro cultures of integrin- and EGFR-overexpressing cancer and human dermal fibroblast cells(control). |
