| 초록 |
The detection of cancer cell via alkaline phosphatase (ALP) activity sensing in coated surface and aqueous state was designed based on specific host-guest and pH-sensitive interaction. The fluorescent probe was prepared by conjugating cross-linked fluorescent nanoparticles (C-FNP), consisting of β-cyclodextrin (β-CD)/boronic acid (BA) and fluorescent hyaluronic acid [FNP(HA)], with fluorescent polydopamine [FNP(pDA)]. The hydrolysis of 4-nitrophenyl phosphate (NPP) into 4-nitrophenol (NP) in the cavity of β-CD by ALP (30-1000 U/L) diminished the fluorescent intensity owing to photoinduced electron transfer (PET). This fluorescence can be recovered by applying pH shock, which triggered release of NP from the fluorescent system due to the cleavage of the diol bond between β-CD and BA. Thus, this system offers promising approach for ALP monitoring and cancer diagnosis, both on coated surfaces and in aqueous states. |
