| 학회 |
한국공업화학회 |
| 학술대회 |
2018년 봄 (05/02 ~ 05/04, 대구 엑스코(EXCO)) |
| 권호 |
22권 1호 |
| 발표분야 |
생체재료_포스터 |
| 제목 |
Rapid and Sensitive of Bacteria Detection using Phenylboronic acid-functionalized Fluorescent Carbon Nanoparticles Switch-on System |
| 초록 |
Boronic acid could be used for the early detection of bacteria through its affinity-binding reaction with diol groups on the bacterial cell wall. Herein, we describe the use of a diol-modified fluorescent probe (DYE) conjugated to a nanosensor consisting of phenylboronic acid-functionalized fluorescent carbon nanoparticles (FNPs) to allow quenching via the Förster resonance energy transfer(FRET) process. In the presence of glucose-containing bacteria, the DYE in the cyclic ester form will be released from the FNP and replaced by the bacterial cell forming a new cyclic boronate ester bond with the nanoparticle, inducing recovery of the fluorescence. Incredibly, the system was most successful in detecting bacteria in the contaminated river water, thus proving it to be a less expensive and more robust affinity biosensor for the detection of contaminating pathogens in various chemoselective ligand-based environments. |
| 저자 |
최청아, 김슬기, 노상규, 원현정, 이기백, 박성영
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| 소속 |
한국교통대 |
| 키워드 |
Phenylboronic acid; Bacteria Detection; Fluorescence switch-On; Fluorescent Carbon Nanoparticles; Förster Resonance Energy Transfer (FRET); Sensor
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| E-Mail |
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