| 초록 |
Parallel Artificial Membrane Permeability Assay (PAMPA) has been widely used as a non-cell based assay to predict the membrane permeability of drug candidates. Despite its simplicity and rapid analysis, the unclear structure of PAMPA membrane still remains a problem. Here, combining freestanding lipid bilayer and absorption spectroscopy, we suggest a better system than PAMPA. Our system uses the artificial membrane which has lipid bilayer structure and allows real-time measurement of membrane transport. Freestanding lipid bilayer is formed when the planar monolayer and the droplet monolayer are brought into contact. Once the lipid bilayer is made, drug molecules start to diffuse from the droplet down to the water through that lipid bilayer. Simultaneous measurement of the UV absorbance during transport enables real-time analysis, which is laborious and not actually conducted in PAMPA. Results are consistent with references and full dynamics data can be obtained with our system. |
