| 초록 |
Methionine, which is one of essential amino acids, can be used for many purposes including food additives, cosmetics, or pharmaceutical reagent. In Escherichia coli, methionine pathway is well known and methionine is synthesized from homocysteine and methyltetrahydrofolate. Because homocysteine comes from TCA cycle and methyltetrahydrofolate can be made from formate and tetrahydrofolate, I’m trying to use glucose and formate simultaneously to produce methionine effectively in Escherichia coli. For this purpose, I performed deletion of ltaE, kbl, aceA and serA genes to make glycine auxotrophic strain, and then performed over-expression of ftfL, mtdA and fch genes that originate from Methylobacterium extorquens AM1 and gcvTHP, lpd genes that originate from Escherichia coli. Through this engineering, Escherichia coli must use formate to synthesize glycine by using reductive glycine pathway. And additional engineering to increase methionine production will be performed. |
