| 초록 |
Efficient target gene delivery into eukaryotic cells is important for biotechnological research and gene therapy. Gene delivery based on proteins and peptideshas recently emerged as a powerful non-viral DNA transfer technique.We investigated the potential use of a recombinant mussel adhesive protein, hybrid fp-151, as a gene delivery material, in view of itsbasic amino acid composition,cost-effective and high-level production in Escherichia coli. After confirming DNA binding affinity, we transfected mammalian cells (human 293T and mouse NIH/3T3) with foreign genes using hybrid fp-151 as the gene delivery carrier. Hybrid fp-151 displayed comparable transfection efficiency in both mammalian cell lines, compared to the widely used transfection agent, LipofectamineTM 2000. Our results indicate that this mussel adhesive protein may be used as a potential protein-based gene-transfer mediator. |
