| 학회 |
한국화학공학회 |
| 학술대회 |
2004년 가을 (10/29 ~ 10/30, 호서대학교(아산캠퍼스)) |
| 권호 |
10권 2호, p.1571 |
| 발표분야 |
생물화공 |
| 제목 |
Semi-continuous Production of Gibberellic Acid by Using immobilized Gibberella fujikuroi B9 |
| 초록 |
Although immobilized cell cultures have potential advantages over suspended cell cultures for the production of secondary metabolites, there are several barriers to overcome for the development of commercial process using immobilized cell system. The one of the critical issues should be maintenance of the catalytic activity of original cells for long time in immobilized cell cultures. We investigated the feasibility of long term production of secondary metabolite, gibberellic acid, in the repeated batch cultures of immobilized fungal cells on celite beads. The optimal concentration of medium component was obtained by central composite design. The volumetric productivity and Yp/s increased as the number of batches increased. We demonstrates that repeated use of immobilized fungal cells is possible without loss of activity of original cells for more than 67 days. Compared with suspended cell cultures, 3-fold higher cell concentration and thus 3-fold higher volumetric productivity were obtained in repeated batch cultures of immobilized cells. |
| 저자 |
김창준1, 장용근2, 전계택3, 정연호3, 이상종4
|
| 소속 |
1경상대, 2한국과학기술원 생명화학공학과, 3강원대, 4(주)에스티알바이오텍 |
| 키워드 |
secondary metabolites; fungal cells; immobilization; repeated batch cultures; gibberellic acid
|
| E-Mail |
|
| 원문파일 |
초록 보기 |
