| 초록 |
Glucose-responsive insulin delivery systems have been proposed as a promising diabetes therapy. The phenylboronic acid (PBA)-derivatives converted to hydrophilic moiety with elevated glucose level play a key role in controlled insulin delivery system due to their better biostability and high biocompatibility. In order to endow glucose-responsiveness to insulin delivery carriers using PBA derivatives, glycol chitosan (GC)/sodium alginate (SA)-poly(L-glutmate-co-N-3-L-glutamylphenylboronic acid) (PGGA) graft polymer double-layered nanogel is synthesized by N-carboxyanhydride (NCA) polymerization and carbodiimide coupling reactions. GC/SA-PGGA double-layered nanogel controllably releases insulin at diabetic glucose levels in vitro, and shows high biocompatibility, determined by cell viability and hemolysis assay. Moreover, controlled insulin release at high glucose levels can be accomplished using GC/SA-PGGA double-layered nanogel in mouse studies. |
