| 학회 |
한국화학공학회 |
| 학술대회 |
2019년 봄 (04/24 ~ 04/26, 제주국제컨벤션센터) |
| 권호 |
25권 1호, p.362 |
| 발표분야 |
생물화공(Biochemical Engineering) |
| 제목 |
Accurate precursor rebalancing for isoprenoid production by fine-tuning of gapA in Escherichia coli |
| 초록 |
To use the 1-deoxy-D-xylulose-5-phosphate (DXP) pathway for isoprenoid production requires equimolar glyceraldehyde 3-phosphate and pyruvate to divert flux toward the isoprenoids. Here, we show that precursor balancing is one of the most important steps for the production of isoprenoids in Escherichia coli. Initially, the synthetic lycopene production pathway as a model system, the amplification of the original DXP pathway were accomplished by using synthetic constitutive promoters and redesigned 5′-untranslated regions (5′-UTRs). Next, phosphoenolpyruvate synthase (PpsA) or glyceraldehyde 3-phosphate dehydrogenase (GAPDH) are tuned to precisely balance the precursors. The results showed that lycopene content in optimal gapA and ppsA variants increased up to 97% and 37% respectively, compared to that of the parental strain. Our results indicate that gapA is the optimal target for precursor balancing to increase biosynthesis of isoprenoids.
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| 저자 |
김민재1, 정주영1, 임재형1, 김세연1, 임대균2, 석주연1, 이승재1, 오민규2, 정규열3
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| 소속 |
1POSTECH, 2고려대, 3postech |
| 키워드 |
생물화학공학 |
| E-Mail |
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| 원문파일 |
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