| 초록 |
MALDI-MS-based quantitative targeted glycomics(MALDI-QTaG) was proposed for sensitive and quantitative analysis of total N-glycans. The derivatization reactions (i.e., amidation of sialic acid and incorporation of a positive charge moiety into the reducing end) increased the linearity (R2>0.99) and sensitivity (limit of detection is 0.5 pmol/glycoprotein) relative to underivatized glycans. Furthermore, the analytical strategy was chromatographic purification-free and non-laborious process accessible to the high-throughput analyses. We used MALDI-QTaG to analyze the N-glycans of α-fetoprotein (AFP) purified from normal cord blood and HCC cell line (Huh7 cells). The total percentages of core-fucosylated AFP N-glycans from Huh7 cells and normal cord blood were 98 and 18 %, respectively. |
