| 초록 |
Chitosans have been proposed as biocompatible alternative cationic polymers that are suitable for non-viral gene delivery. However, the transfection efficiency of chitosan-DNA nanoparticles is still very low due to low polyelectrolyte complexation. In this study, a chitosan with a guanidinium side group which improves cell membrane-penetrating properties as well as transfection efficiency was succesfully synthesized by chemical modification, using 3,5-dimethylpyrazole-1-carboxamidine nitrate salt under weak basic conditions. The formation and guanidinylation of chitosan were confirmed by NMR spectroscopy and the composition and degree of substitution of guanidino in modified chitosan were also estimated by elemental analysis. The physicochemical properties of modified chitosan were evaluated by particle size measurement and zeta potential assay. Also, the transfection efficiency of modified chitosan were investigated in various cancer cell lines |
