| 초록 |
In continuation of our previous studies on blood compatibility profiles of anion-substituted poly(vinyl alcohol) membranes in which hydroxyl groups have been replaced with carboxymethyl (C-PVA) and sulfonyl groups (S-PVA), the activation of complement components and the changes in white cell and platelet have been studied in vitro and compared with those of unmodified PVA, Cuprophane, and low-density polyethylene (LDPE). Complement activation of fluid phase components, C3a, Bb, iC3b, and C5b-9, and of bound phase, C3c, C3d, and SC5b-9, were assessed by ELISA and Western blot analysis, respectively. The changes in the number of white cell and platelet followed by complement activation was counted by Coulter counter. C- and S-PVA activated less extent of C3 than PVA that can be attributed to the diminished level of surface nucleophiles of samples. In addition, C- and S-PVA exhibited increased inhibition activity of Bb production, resulting in decrease in the extent of C5 activation. Consequently, owing to reduced activation of C3 and C5, C- and S-PVA samples showed marked decrease in SC5b-9 levels in plasma. It was also found that negatively charged sulfonate and carboxylate groups of the samples adsorbed large extents of positively charged anaphylatoxins, C3a and C5a, due to strong electrostatic attraction, resulting in an inhibition of chemotaxis and activation of leukocytes. The inhibition ability of complement production together with binding ability of anaphylatoxins of C- and S-PVA samples lead to a marked decrease in lysis of leukocytes as well as activation of platelet. |
