| 학회 |
한국고분자학회 |
| 학술대회 |
2007년 가을 (10/11 ~ 10/12, 일산킨텍스) |
| 권호 |
32권 2호 |
| 발표분야 |
생체모방형 고분자재료(의료용 고분자 부문위원회) |
| 제목 |
Galactosylated Chitosan-graft-Polyethylenimine as a Gene Carrier for Hepatocyte Targeting |
| 초록 |
Chitosans have been proposed as alternative biocompatible cationic polymers for non-viral gene delivery. However, the low transfection efficiency and low specificity of chitosans needs to be addressed prior to clinical application. In this study, a galactosylated chitosan-graft-polyethylenimine (GC-g-PEI) copolymer was prepared by an imine reaction between periodate-oxidized GC and low molecular weight PEI. The molecular weight and composition were characterized using GPC-MALS and 1H NMR), respectively. GC-g-PEI/DNA transfection efficiency into HepG2, which has asialoglycoprotein receptors (ASGPR), was higher than that into HeLa without ASGPR, indication of increased cell-type specificity. GC-g-PEI/DNA complexes also transfected liver cells in vivo following i.p. administration more effectively than PEI 25K alone. These results suggest that GC-g-PEI can be used in gene therapy to improve transfection efficiency and hepatocyte specificity in vitro and in vivo. |
| 저자 |
강호림1, 권정택2, 김유경1, 로히다스1, 김은미3, 정환정3, 조명행4, 조종수1
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| 소속 |
1서울대, 2서울대 수의과대, 3전북대 핵의학과, 4서울대 수의학과 |
| 키워드 |
gene therapy; non-viral vector; targeting gene delivery; galactosylated chitosan-graft-PEI
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| E-Mail |
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