| 학회 |
한국화학공학회 |
| 학술대회 |
2018년 가을 (10/24 ~ 10/26, 대구 EXCO) |
| 권호 |
24권 2호, p.1463 |
| 발표분야 |
생물화공(Biochemical Engineering) |
| 제목 |
Metabolically Engineered Escherichia coli for High-Level Production of Astaxanthin |
| 초록 |
Astaxanthin is a reddish keto-carotenoid which acts as powerful antioxidant. Here, we report metabolically engineered Escherichia coli for high-level and high productivity astaxanthin production. First, the crt genes (crtE, crtY, crtI, crtB, and crtZ) from Pantoea ananatis and the truncated BKT gene (trCrBKT) from Chlamydomonas reinhardtii were introduced. Eight different fusion tags were tested on trCrBKT for stable expression and efficient guiding to the membrane. The signal peptide (OmpF and TrxA) were tagged to trCrBKT and 12.90 mg/L of astaxanthin was produced, 2.08-fold higher than without tags. Culture condition optimization increased the production up to 332.23 mg/L by fed-batch cultivation. Further improvement by overexpressing the ispD and ispF genes, identified by in silico FVSEOF, produced 377.10 mg/L of astaxanthin with 9.20 mg/L/h productivity. The titer increased to 432.82 mg/L with a productivity of 9.62 mg/L/h when IPTG was reduced to from 1 mM to 0.5 mM. Hok/sok system was introduced to improve plasmid stability and this strain produced 385.04 mg/L of astaxanthin with a productivity of 7.86 mg/L/h.(NRF-2012M1A2A2026556 and NRF-2012M1A2A2026557) |
| 저자 |
Prabowo Cindy Pricilia Surya1, 박선영2, Robert M. Binkley1, 김원준2, 이문희1, 이상엽2
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| 소속 |
1Metabolic and Biomolecular Engineering National Research Laboratory, 2KAIST |
| 키워드 |
생물화공 |
| E-Mail |
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| 원문파일 |
초록 보기 |
