| 학회 |
한국공업화학회 |
| 학술대회 |
2019년 봄 (05/01 ~ 05/03, 부산 벡스코(BEXCO)) |
| 권호 |
23권 1호 |
| 발표분야 |
나노_포스터 |
| 제목 |
Multiplexed and time resolved immuno-biosensing of histamine by dual layer self-assembled liposomal nano-fluorophores |
| 초록 |
Histamine as neurotransmitter, plays an important role in regulating the biological circadian rhythm and wakefulness in human health. Liposome-based multiplexed dual-layered assembled lipo-immuno-nanofluorophore biosensing was developed as a suitable biosensor device, which integrates immuno-liposomes in dual layered sandwich immunoassay format. Method 1 based on a dual-layered single wash procedure, which had a detection limit of 10 ppb and Method 2 was based on a dual layered one-by-one wash procedure and had a detection limit of 2–3 ppb that required 2 h 30 min to perform. Method 2 was chosen for further validation. The feasibility of developed multiplexed sensing was tested in histamine contaminated fresh mackerel fish, and canned fish (tuna), which showed a similar detection range but with low amplified fluorescence signal intensities and sufficient levels of histamine recovery from fresh mackerel (73.5-99.98%), canned tuna (79.08–103.74%). |
| 저자 |
오서영1, Shruti Shukla2, Vivek K. Bajpai2, Imran Khan1, 전태준1, 이은선3, 오미화3, 한영규2, 허윤석1
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| 소속 |
1인하대, 2동국대, 3National Institute of Animal Science |
| 키워드 |
Liposome immunosensor; Signal amplifiers; Fluorescence quenching; Lipo-immuno-nanofluorophore; Histamine
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| E-Mail |
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