| 초록 |
Since several decades, many studies for mimicry of a living organism have been done owing to its distinctive. Yet, few have been attempted to replicate the unique properties. Here we first present a cell’s nucleus with the ability of a transcript decoder for an enhanced mRNA level. It is simply created by enzymatically crosslinking a gene with DNA origami crosslinkers resembling a histone as a gene holder. After further treatment with lipid bilayers and successive transcription factors, it effectively promoted the expression of a target RNA transcript. In comparison with other control groups (e.g., ones without either a gene or a crosslinker), it showed much stronger emission of encoding RNAs tested. Once internalized by target cell (e.g., MCF-7), it ensued a higher and longer expression of GFPs. It successfully replaced the older and restored the system. The artificial cell nucleus mimicry may be a new archetype for advanced gene therapy. |
