| 초록 |
Vascular Tissue engineering by in vitro bioreactor system has drawn interest in its medical society due to its simulation of pulsatile flow application. We induced the media tissue regeneration with porcine smooth muscle cells on the inside surface layer of the hybrid vascular scaffolds, which was formed with two biodegradable porous biodegradable poly(lactide-co-glycolide) layers and one non-biodegradable polytetrafluoroethylene, by employing a bioreactor system with controlled pulsatile flow, in vitro temperature and pH. The hybrid scaffold was in vitro cultured under static and dynamic conditions after collagen coatings on its biodegradable surfaces. The characterizations of the scaffolds were performed with scanning electron microscopy for the observation of morphologies and immuno-histochemistry for verification of distinctively regenerated tissues dependent on culture conditions. While flow-directed surfaces demonstrated cellular orientation by pulsatile flows compared to the static conditions, media tissue was generated by replacing the biodegradable polymer layers along their time-dependent degradation. |
