| 초록 |
We present the kinetic model which describes the heterogeneous reaction system for the production of N-carbamoyl-D-p-hydroxyphenylglycine (NCHPG) from poorly-soluble substrate, 5-(4-hydroxyphenyl)hydantoin (HPH) by using mass-produced D-hydantoinase. The model involves dissolution of the solid substrate, enzymatic conversion of the D-form substrate, racemization of the L-form substrate to the D-form, and deactivation of the enzyme. In the case of the whole cell enzyme, transfer of the dissolved substrate and product through the cell membrane was considered. Performance of the reaction systems using free and whole cell enzymes were predicted and compared with the experimental results. Factors affecting the kinetics of the heterogeneous reaction systems were also analyzed based on the kinetic model.
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