| 초록 |
In this study, we fabricated a multilayered fibrous scaffold capable of controlled release of multiple growth factors for scitaic nerve regeneration. The scaffold consists of three layers prepared by electrospinning, where the first layer consists of PCL-aligned electrospun nanofibers for cell attachment. The second and third layers are fabricated using PLGA 6535 and 8515, respectively. The resultant three nanofiber layers were stacked and fixed by hydrogel micropatterns at both ends of the scaffold, which also facilitated the surgical handling of the scaffold. The PLGA layers acted as reservoirs for growth factors NT-3, BDNF, and PDGF. The different biodegradation rate of each layer enabled the controlled release. In a rat model, the injured nerve was rolled up with the scaffod loading growth factors, and behavior tests were performed. The analysis revealed that the fast release NT-3 and BDNF and subsequent slow release of PDGF proved to be the most efficient in neural regeneration. |
