| 초록 |
We herein describe a novel fluorescent method for the sensitive and selective detection of adenosine by utilizing hydrolysis reaction for s-adenosylhomocysteine (SAH) catalyzed by s-adenosylhomocysteine hydrolase (SAHH). In this study, DNA-Ag/Cu nanoclusters complex was served as a fluorescent signaling materials, which is quenched by thiol functional group (-SH). When SAHH catalyzes the hydrolysis reaction of SAH, homocysteine containing thiol group is generated, which causes reduced fluorescence signal. In the presence of adenosine, however, the hydrolysis reaction is significantly inhibited resulting less exposure of thiol group of homocysteine, which finally brings about recovery of the fluorescence. In other words, fluorescence signal decreases with the increased concentration of adenosine. By employing this strategy, SAHH activity was reliably measured with DNA-Ag/Cu nanoclusters and also adenosine was detected sensitively with a limit of detection of 0.15 μM. In addition, adenosine was detected with the high selectivity over any adenosine derivatives such as AMP, ADP and ATP. |
