| 학회 |
한국화학공학회 |
| 학술대회 |
2013년 봄 (04/24 ~ 04/26, 광주 김대중컨벤션센터) |
| 권호 |
19권 1호, p.345 |
| 발표분야 |
생물화공 |
| 제목 |
Mussel adhesive protein-based whole cell array biosensor for environmental monitoring of organophosphorus compound |
| 초록 |
A novel whole cell-based array biosensor for detection of neurotoxic paraoxon was developed by immobilizing Escherichia coli expressing organophosphorus hydrolase in periplasmic space on mussel adhesive protein (MAP)-coated 96-well microplate. The effect of MAP as a cell immobilizing agent was evaluated by comparing with simple physical adsorption mehtod. Paraoxon-degradable activity and fluorescence microscopy studies revealed that the use of MAP has advantages of increasing cell immobilizing efficiency and enhancing stability of immobilized cells compared to simple physical adsorption-based whole cell biosensor. Scanning electron microscopy analysis also showed that MAP effectively immobilized cells on surface without pretreatment steps. The whole cell-based array biosensor could detect as low as 5 μM paraoxon with high reproducibility and the detection range of the biosensor was 5–320 μM. In addition, the whole cell biosensor showed good long-term storage and multiple-use stability. Collectively, our proposed whole cell array biosensor is suitable tool for rapid and sensitive detection of harmful organophosphorus compounds. |
| 저자 |
김창섭, 최봉혁, 서정현, 차형준
|
| 소속 |
포항공과대 |
| 키워드 |
Whole cell array biosensor; Mussel adhesive protein; Cell-immobilizing linker; Organophosphorus hydrolase; Escherichia coli
|
| E-Mail |
|
| 원문파일 |
초록 보기 |
