| 초록 |
Exosomes have been attracted significant interests due to its biological importance in that they play a substantial role in intercellular communication and contain various biomolecules. However, due to their difficulties in effective capture and detection, further application of exosomes has been challenged. For detection of EVs, we fabricated liposomal biosensor based on the polydiacetylene (PDA) which is a conjugate polymer that has been widely used to sensing applications based on its unique optical properties. To confer the selectivity to the sensory material, the CD9 antibodies are attached to the PDA liposomes and phospholipid molecules. Signal analysis for exosome detection and quantification was performed by observing colorimetric changes triggered by the ligand-receptor interaction of PDA vesicles. Visual, UV-visible, and fluorescence spectroscopic methods were used to obtain signals from the PDA lipid immune-sensor with a detection limit of 3×10^8 vesicles/mL |
