| 학회 |
한국고분자학회 |
| 학술대회 |
2015년 가을 (10/06 ~ 10/08, 대구컨벤션센터(EXCO)) |
| 권호 |
40권 2호 |
| 발표분야 |
의료용 고분자 부문위원회 II |
| 제목 |
Ultra-sensitive detection of antigen using functionalized liquid crystal droplets |
| 초록 |
The orientational variation of liquid crystal molecules in LC microdroplets in response to IgG(antigen) has been used to develop ultra-sensitive biosensor for label detection of IgG in biological matrices. To prepare LC microdroplets based biosensor, 4-cyano-4’-pentyl biphenyl(5CB) liquid crystal microdroplets anchored with anti-IgG(antibody) were prepared using sodium dodecylsulfate(SDS) as a mediator and poly(styrene-b-acrylic acid) amphiphilic block copolymer(PS-b-PA) used as a modifier of LC/water interfaces. The anti-IgG anchored LC microdroplets with a size variation have been used for ultra-sensitive detection of IgG within the concentration range from 20-1000 ng/mL with a response time of 30 minutes in PBS solution. The anti-IgG anchored LC microdroplets found useful in detection of IgG in solution containing 10% FBS or 10 % blood plasma. However, the response time of anti-IgG anchored LC microdroplets in detection of IgG in 10% FBS or 10% plasma was delayed to 90 minutes. |
| 저자 |
위에 환, 강인규, 이규배
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| 소속 |
경북대 |
| 키워드 |
liquid crystal microdroplet; amphiphilic block copolymer; biosensor; immunoassaying
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| E-Mail |
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