| 초록 |
Synthetic biology aims to develop engineering-driven approaches to the programming of cellular functions that could yield transformative technologies. Despite many advances in synthetic gene circuits, challenges remain for scaling up the complexity of these networks due to the limited number of composable parts and substantial resource requirements. Here, we report a strategy for constructing RNA-only nanodevices to evaluate complex logic in living cells. Such ‘ribocomputing’ systems are composed of de novo designed parts and operate via predictable and designable base-pairing rules, allowing for effective in silico design of computing devices with prescribed configurations and functions in complex cellular environments. We demonstrate that these ribocomputing devices in Escherichia coli can evaluate four-input AND, six-input OR, and a complex 12-input logic expression. |
