| 초록 |
A successful delivery of interleukin (IL)-15 to natural killer (NK) cells and a subsequent activation of anticancer efficacy could be a promising immuno-cancer therapy. For the protection of IL-15 with maintaining its bioactivity, coacervate (Coa) (a complex of (1) methoxy polyethylene glycol-poly(ethylene arginylaspartate diglyceride) (mPEG-PEAD), (2) heparin, and (3) cargo IL-15) were developed. A release profile of IL-15 from Coa could be controlled by PEGylation to PEAD. After 8 days of culture with IL-15 loaded Coa, proliferation of NK cells was enhanced compared to bolus IL-15 group. In addition, mRNA expression related to anticancer efficacy of NK cells were upregulated by priming. Furthermore, when NK cells co-cultured with multiple cancer cells, Coa group exhibited facilitated cancer killing efficacy than bolus IL-15 group. In summary, coacervate-mediated exogenous IL-15 delivery could be an effective NK cell priming technique and be utilized for a cancer-immuno therapy. |
