| 초록 |
Poly(ethylene arginyl aspartate diglyceride) (PEAD) polycation is widely used to prepare coacervate particles by electrostatic complexation with an anionic heparin (HEP) in aqueous environments, for controlled release of therapeutic proteins. Although coacervates using PEAD have randomized complexes, mPEGylated PEAD and HEP polyplexes have a certain “coacersome” structure. The mPEG moiety, assembles on the surface, creates a steric barrier avoiding aggregation. The coacersomes maintain initial spherical morphology and size despite of competing ions. Additionally, the coacersomes exhibit biocompatibility toward human dermal fibroblasts, a high loading efficiency for encapsulation of bone morphogenetic protein 2 (BMP-2), and a sustained release profile. In addition, BMP-2-loaded coacersomes increased osteogenic differentiation of human mesenchymal stem cells. The developed coacersome structures have the potential to be utilized as effective carriers for therapeutic protein delivery. |
