| 학회 | 한국화학공학회 |
| 학술대회 | 2012년 봄 (04/25 ~ 04/27, 제주 ICC) |
| 권호 | 18권 1호, p.494 |
| 발표분야 | 생물화공 |
| 제목 | Precise multiplex expression analysis using multiplex ligation-dependent probe amplification based on conformation-sensitive capillary electrophoresis |
| 초록 | Quantification of mRNA provides information crucial for various biological studies. Real-time PCR is known to be the most accurate method for quantifying mRNA, and thus represents the state-of-the-art for gene expression analysis. However, the use of real-time PCR for mRNA quantification is limited to a single target per analytical run because of reductions in quantification power and limitations of fluorescence dyes associated with multiplex applications. Multiplex ligation-dependent probe amplification (MLPA) is an alternative multiplex analysis method. However, MLPA has not been widely used for expression analysis because it uses DNA-size-dependent electrophoretic separation, which complicates probe design process and compromises accuracy of the analysis. In this study, we developed a new version of MLPA, which uses a conformation-sensitive electrophoretic separation. We have demonstrated that our method could be used to monitor expression of genes in Escherichia coli, Caenorhabditis elegans and Arabidopsis thaliana. |
| 저자 | 신기원, 황희성, 정보람, 나정경, 정규열 |
| 소속 | 포항공과대 |
| 키워드 | expression analysis; cooformation-sensitive capillary electrophoresis; multiplex ligation-dependent probe amplification |
| VOD | VOD 보기 |
| 원문파일 | 초록 보기 |
