| 초록 |
In this study, we constructed a fusion strain, SP4, which has inaA::luxCDABE, based upon real-time quantitative PCR analysis when E. coli BL21(DE3) was exposed to ferulic acid and coumaric acid. To characterize this strain, we observed the responses to various concentrations of ferulic acid and compared inaA expression levels with many other compounds identified within hydrolysates. From the tests, it was shown that the aromatic acids and aldehydes caused induction of inaA expression while acetic acid and furfural did not affect the inaA expression. Also, we transformed the plasmid into several E. coli strains and mar mutant strains and monitored the responses from them, in order to see the effects from the difference of genetic backgrounds. Even though the induction level of marA- strain was lower than that of marA+ strain, the marA- strain still showed significant induction, suggesting some other regulatory mechanisms for inaA expression. Finally, we applied this biosensor to test actual hydrolysate samples and monitor the degradation of benzoate. |
