| 초록 |
Systems biology understands a biological system including an organism as a whole by assessing relevant biological components at the genome scale. This approach often includes in vivo experimental measurements of biomolecules such as DNA, RNA, or protein and/or in vitro computational calculation/simulation. As examples of systems biology approaches, regulation of bacterial metabolism was investigated. We generated and integrated ChIP-exo and RNA-seq data to identify Cra regulon of carbon metabolism. An integrated metabolic-regulatory network was formed by including experimentally-derived regulatory information and a genome-scale metabolic network reconstruction. Applying analysis methods of systems biology to this integrated network showed that Cra enables the optimal bacterial growth on poor carbon sources by redirecting and repressing the glycolysis flux, by activating the glyoxylate shunt pathway, and by activating the respiratory pathway. |
