| 학회 | 한국화학공학회 |
| 학술대회 | 2007년 가을 (10/26 ~ 10/27, 한국과학기술원) |
| 권호 | 13권 2호, p.1694 |
| 발표분야 | 생물화공 |
| 제목 | Dispersed magnetic silica nanoparticles with immobilized metal affinity ligands for his-tagged enzyme adsorption |
| 초록 | Silica-coated magnetite(Fe3O4) nanoparticles with immobilized metal affinity ligands were prepared for histidine-tagged enzyme adsorption. magnetite nanoparticles were synthesized by co-precipitating Fe2+ and Fe3+ in an ammonia solution. Then silica was coated on the Fe3O4 nanoparticles using a sol-gel method to obtain magnetite silica nanoparticles. The condensation product of 3-Glycidoxypropyltrimethoxysilane (GLYMO) and iminodiacetic acid (IDA) was immobilized on them and after charged with Cu2+, the magnetic silica nanoparticles with immobilizes were applied for the adsorption of histidine-tagged lipase. TEM showed that the magnetic silica nanoparticles with an average size of 70nm were well dispersed image. X-ray diffraction showed the spinel structure for the magnetic particles coated with silica. the stability of the free and the immobilized histidine-tagged lipase was studied under various conditions. |
| 저자 | 김혜주, 황길수, 황상필, 안익성 |
| 소속 | 연세대 |
| 키워드 | magnetic nanoparticles; silica coating; metal affinity; enzyme adsorption |
| 원문파일 | 초록 보기 |
