| 초록 |
In this study, the effect of different concentrations (0.05 0.15, 0.25, 0.35, 0.45% w/v) of Hyaluronic acid (HA) on the prevention of post-surgical peritoneal tissue adhesion was investigated in a rat model of cecal abrasion. An effective injectable adhesion barrier was formulated comprising methyl cellulose, HA, polyethylene glycol, and plant derived tempo oxidized cellulose nanofibre, with unique inverse thermal gelling, high stability and anti-adhesive properties. Methyl cellulose successfully converts the solution into solid gel at body temperature (37oC). Water-soluble tetrazolium (WST) salt assay showed that the hydrogels were nontoxic to rat bone marrow mesenchymal stem cells (RBMSCs) and L929 fibroblast cells. In addition, higher HA concentrations increased degradation rat of hydrogel. In vivo study involving rat model of cecal abrasion confirmed that the 0.25% HA containing hydrogel could easily adhered to the damaged peritoneal surfaces, and gradually degraded within 14 days with optimum healing. Thus, highly functional 0.25% HA combined cellulose polymers blended effective and safe injectable adhesion barrier was fabricated without any crosslinking agent and can be applied as novel therapeutic potential to prevent post-surgical peritoneal adhesions. |
