| 학회 |
한국화학공학회 |
| 학술대회 |
2002년 가을 (10/24 ~ 10/26, 서울대학교) |
| 권호 |
8권 2호, p.3905 |
| 발표분야 |
생물화공 |
| 제목 |
Study of biosynthesis of gentamicin and characterization of 2-deoxyscylloinosose synthase from Micromonospora purpurea |
| 초록 |
Gentamicin is an interesting 2-deoxystreptamine containing antibiotics produced by Micromonospora purpurea. Recently, we have cloned and sequenced the genes involved in the biosynthesis of 2-deoxystreptamine, a sugar moiety retained in all gentamicins. To prove the functionalities, 2-deoxyscylloinosose synthase, a key enzyme required for the biosynthesis of DOS was manipulated. The gene was over expressed in E. coli in the soluble form and the activity was studied in vitro. In a parallel experiment, the gene was inactivated and its function was studied in vivo by analyzing the metabolites produced by the mutants. In addition, the function of gentamicin resistant ribosomal methyltransferase was also verified. |
| 저자 |
Madan Kumar Khare1, Young-Ho Moon2, Jae-jeong Kim, Jin Suk Woo, Joo Ho lee, Hei Chan Lee, Kwangkyoung Liou, Jae Kyung Sohng
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| 소속 |
1Institute of Biomolecule Reconstruction, 2SunMoon Univ. |
| 키워드 |
gentamycin; biosynthetic pathway; 2-deoxyscylloinosose synthase
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| E-Mail |
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| VOD |
VOD 보기 |
| 원문파일 |
초록 보기 |
