| 학회 | 한국화학공학회 |
| 학술대회 | 2008년 봄 (04/23 ~ 04/25, 제주ICC) |
| 권호 | 14권 1호, p.469 |
| 발표분야 | 생물화공 |
| 제목 | Highly sensitive RNA chip to detect viable bacteria |
| 초록 | The presence of these bacteria such as Escherichia coli and Salmonella in food or water indicates that bacterial contamination has occurred and consumers can be exposed to enteric pathogens. Especially, detection of RNA in bacteria provides the sophicated measurement of viable pathogen. Therefore, we developed highly sensitive RNA chip to detect viable bacteria by adding preconcentration process prior to amplification in order to increase low concentration of RNA. Concentrated RNA was amplified using nucleic acid sequence-based amplification (NASBA), that is isothermal amplification method. Finally progress of preconcentration and amplification was applied to one-chamber RNA Chip. We developed new single RNA chip having whole process from cell lysis to amplification in a chip. It provides fast and simple method to detect low concentration of pathogen. Acknowledgment : This work was supported by the Korea Science and Engineering Foundation (KOSEF) grant funded by the Korea government(MOST) (No. M10755160002-07N5516-00210) |
| 저자 | 김희정1, 김정호2, 박정환2, 민준홍2 |
| 소속 | 1서강대, 2경원대 |
| 키워드 | RNA chip; NASBA; micro bead |
| 원문파일 | 초록 보기 |
