| 학회 |
한국공업화학회 |
| 학술대회 |
2021년 가을 (11/03 ~ 11/05, 대구 엑스코(EXCO)) |
| 권호 |
25권 2호 |
| 발표분야 |
포스터-생체재료 |
| 제목 |
Enhanced Particle Rinsing Capabilities for Efficient microRNA Detection via Highly Magnetized Encoded Hydrogel Microparticles |
| 초록 |
Encoded hydrogel microparticles conjugated with DNA probes are advantageous for high-performance microRNA (miRNA) detection in terms of specificity, sensitivity and multiplex detectability. However, particle rinsing steps in the assay procedure present challenges for rapid and efficient detection. To overcome this limitation, we encapsulated magnetic nanoparticles to microparticles to reduce the rinsing steps and duration via magnetic separation. We attached DNA probes targeting three types of miRNAs related to preeclampsia to magnetically encoded hydrogel microparticles by post-synthesis conjugation and achieved sensitivity comparable to that of conventional nonmagnetic particles. Furthermore, we conducted a triplex detection to prove the multiplexed detectability. |
| 저자 |
장우경1, 봉기완1, 김지우1, 문석준1, 김선민2
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| 소속 |
1고려대, 2서울특별시 보라매병원 / 서울대 |
| 키워드 |
hydrogel; microparticles; preeclampsia; microRNA detection
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| E-Mail |
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