| 초록 |
We observed the chemotactic behavior of a motile microalga, Chlamydomonas reinhardtii, in a flow-based microsystem. According to the previous studies, C. reinhardtii utilizes carbon dioxide (CO2) and bicarbonate ion (HCO3-) as carbon sources at the same time. Firstly, we devised a microfluidic system which has three inlets for stable concentration gradient and 9 outlets for statistical analysis of the cell distributions. Pressure-driven flows were applied in the device by three syringe pumps and the cell numbers were counted at the entrance part of each outlet. We digitized the chemotactic responses of each strain by analyzing cell distributions. As a result, well-growing mutants had a tendency to prefer higher concentration. In other words, the chemotactic responses of well-growing mutants showed higher values. In addition, the correlations among the chemotactic response, △OD and Y(II) showed meaningful relationships. |
