| 초록 |
In this study, we proposed two different biosensor platforms that were fabricated with hydrogel micropatterns incorporating nanomaterials. First, we developed a novel silver-based MEF biosensing platform that consisted of poly(ethylene glycol)(PEG) hydrogel microstructures entrapping silica-coated AgNPs (Ag@SiO2). AgNPs were coated with different thickness of silica to optimize the MEF effects. Second, describes the design and fabrication of a microdevice as a new platform for highly sensitive MMP-9 detection. For enhanced MMP-9 detection, fluorescently labeled MMP-9-specific peptides were immobilized onto an electrospun nanofiber matrix that was framed with hydrogel micropatterns via photolithography. Due to the huge surface area of the nanofiber and small dimensions of the microsystem, a faster response time (30 minutes) and lower detection limit (10 pM) could be achieved. |
