| 초록 |
In order to extract the curcuminoids from turmeric efficiently, The extracted sample were simultaneously separated and analyzed by commercial C18 reversed-phase high performance liquid chromatography using water and acetonitrile as mobile phase with isocratic elution mode. From the experimental results, the optimum mobile phase composition of water/acetonitrile is 60/40 vol% using Knauer RP-column. It is evident that the amount of curcuminoids extracted (extraction time 4 h) by 100% MeOH was higher than any aqueous MeOH composition. The correlation coefficients were greater than cucumin (1) 0.9985, demethoxycurcumin (DMC) (2) 0.9982, and bisdemethoxycurcumin (BDMC) (3) 0.9990. The LC-MS data provide molecular weight information. So, the optimum operating conditions were experimentally determined. ACKNOWLEDGEMENTS : This work was supported by the “Study on drug efficacy enhancement using bioconversion for herbal medicines” (K12050) project. |
