| 초록 |
MicroRNAs play critical roles in controlling various cellular processes, and the expression levels of individual miRNAs can be considerably altered in pathological conditions such as cancer. Especially, specific miRNAs of brain have important roles in neuronal differentiation, development, plasticity and induced neurological disorders. Therefore, accurate quantification and visualization of miRNA at the single-cell level will lead to a better understanding of miRNA function. In situ detection of intracellular miRNAs requires simple sample preparation, without cell lysis, and provides additional information on the subcellular localization of miRNAs. However, conventional tools are not easy to detect high spatial localization and sensitive detection of miRNAs. Therefore, a new approach for miRNA detection tool is atomic force microscopy. Here, we present a direct and ultrasensitive method for miRNA detection using Force-based Bio-AFM. |
