| 초록 |
Deoxyribose nucleic acids with the consecutive number of guanine can form a G-quadruplex structure in the presence of monovalent ions. G-quartets, consisting of four guanines connected by a hoogsteen hydrogen bond, are stacked to form a G-quadruplex. The representative DNA sequence of forming G-quadruplex is known that TBA, HTG, ARGO100, PS2.M, T2. We investigated the structural conversion by heavy metal ion using these sequences. Using Circular dichroism, G-quadrupelx structure for antiparallel structure has shown that negative band at 260nm, positive band at 290nm in CD Spectra. For parallel structure, it shown that positive band at 260nm, positive band at 290nm. However, the G-quadruplex Induced by pb2+ ions convert to another structure, having a negative band around 275~290nm and shifted from positive band 290nm to 315nm. We will investigate that it can detect lead in vivo through G-quadruplex structural conversion. |
