| 학회 |
한국고분자학회 |
| 학술대회 |
2014년 가을 (10/06 ~ 10/08, 제주 ICC) |
| 권호 |
39권 2호 |
| 발표분야 |
의료용 고분자 부문위원회 |
| 제목 |
Development of mannan-decorated thiolated Eudragit microspheres for an efficient nasal vaccine delivery |
| 초록 |
We decorated thioated Eudragit microspheres with mannan for targeting mannose receptors (MR) in antigen presenting cells (APCs) to obtain the systemic and mucosal immune responses through nasally administrated ovalbumin (OVA) as a model vaccine. Mannan-decorated thiolated Eudragit microspheres (Man-TEM) and thiolated Eudragit microspheres (TEM) were prepared using a double emulsion method (W/O/W) and characterized them physicochemically. The APC cell lines were used to validate and clarify the uptaking efficiency and mechanism by CLSM and FACS analysis. As a result, Man-TEM had higher uptaking ability than TEM, indication of MR receptor-mediated endocytosis mechanism. Nasal vaccination of OVA-loaded Man-TEM in mouse showed higher levels of serum IgG and mucosal IgA than the TEM-treated group due to the tageting ability of mannose in the Man-TEM. These results suggest that Man-TEM may be a promising candidate for a nasal vaccine delivery system to elicit systemic and mucosal immunity. |
| 저자 |
이혜선, 강상기, 최윤재, 조종수
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| 소속 |
서울대 |
| 키워드 |
Mannose receptor targeting; Thiolated Eudragit microspheres; Antigen presenting cells(APC); Nasal vaccine
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| E-Mail |
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