| 학회 |
한국공업화학회 |
| 학술대회 |
2021년 가을 (11/03 ~ 11/05, 대구 엑스코(EXCO)) |
| 권호 |
25권 2호 |
| 발표분야 |
포스터-나노 |
| 제목 |
Synthesis of DNA-directed synthesized gold nanoparticle for application of DNA point mutation detection |
| 초록 |
Distortions in the binding affinity of site-specific protein to DNA are associated with genetic diseases, but high-fidelity methods for establishing a consensus rank of protein affinity for mutant DNA—particularly single-point mutations—for the purpose of early diagnosis are lacking. Here we report non-labeling endpoint detection and dynamic analysis of the binding of the mismatch repair initiation protein MutS to the eight most common single-point mutations in BRCA1 using a single-nanoparticle microfluidic platform comprising gold-bridged nanoparticles produced by direction-specific synthesis that can directly transduce information on the MutS-DNA interaction. Mutant DNA was classified into four types based on MutS binding affinity for various point mutations. This powerful method can be used to profile mutant DNA, analyze protein-DNA interactions, and elucidate the mechanisms of gene regulation in disease states. |
| 저자 |
최영재, 김수현, 송소진, Xingyi Ma, 심상준
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| 소속 |
고려대 |
| 키워드 |
DNA-directed synthesis; plasmonic; DNA point mutation; nanobiosensor
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| E-Mail |
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