| 학회 |
한국고분자학회 |
| 학술대회 |
2018년 가을 (10/10 ~ 10/12, 경주컨벤션센터) |
| 권호 |
43권 2호 |
| 발표분야 |
대학원생 구두발표(발표15분) |
| 제목 |
Protein Nanocapsules by Enzymatic Removal of a Polymer Template |
| 초록 |
Protein nanocapsules are potentially useful as functional nanocarriers because of their hollow structures, high biocompatibilies, and the intrinsic activity of their protein constituents. However, developing a facile method for the preparation of protein nanocapsules that retain their protein activity has been challenging. Here we describe the preparation of protein nanocapsules through the enzymatic removal of polymer templates. Nickel(II) nitrilotriacetic acid-end-functionalized poly(lactic acid) was introduced as a polymeric template to immobilize hexa-histidine-tagged green fluorescence proteins with consistent orientation. Following protein cross-linking and core-degradation, AFM and NMR study indicated the formation of protein hollow structures. We also confirmed the orientation control and the activity preservation of the protein after capsule preparation. |
| 저자 |
이채연1, 황아란1, Leeja Jose1, 박지현2, 송재광2, 백현종1
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| 소속 |
1부산대, 2한국화학(연) |
| 키워드 |
Nitrilotriacetic acid (NTA); biodegradable polymer; non-covalently connected micelle; enzymatic core degradation; protein nanocapsule; orientation control of protein
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| E-Mail |
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