| 초록 |
To increase anti-tumor immunity, we have designed and synthesized immunomodulatory hybrid nanoconjugates (HNCs) based on polymer nanocomposites containing imaging tracers conjugated with CpG ODNs (as a TLR9 ligand) and STAT3 siRNAs (as an immunosuppressive gene silencer).These HNCs efficiently targeted immune cells, induced TLR activation, and silenced immunosuppressive genes. Simultaneous delivery of STAT3 siRNAs and CpG ODNs to the tumor microenvironment caused the inhibition of STAT3 along with activation of DCs by CpG ODNs, and their antitumor effects were found to be synergistic. For the effective mucus delivery of a viral antigen that can induce strong mucosal immunity, we also successfully generated a novel anti-influenza vaccine system combining conserved matrix protein 2 (sM2) and stalk domain of hemagglutinin (HA2) fusion protein (sM2HA2) and mucoadhesive polymer–based vaccine adjuvant systems. Intranasal co-administration of sM2HA2 and the combination adjuvant was able to induce a high degree of protective mucosal, systemic, and cell-mediated immune responses. |
