| 초록 |
Fluorescence imaging is widely used in biological studies due to its ease of manipulation, safety, and relatively low cost. For the imaging requiring penetration of light into the living tissues, the usage of fluorophores with their excitation and emission wavelengths in the near-infrared (NIR) region is beneficial to minimize the issues of autofluorescence and penetration depth. As our continued effort to develop high-performance fluorescent probes for in vivo applications, here we report a photochromic diarylethene-crosslinked dendritic nanocluster as the biocompatible and water-soluble reversible fluorescence photoswitch, with its emission in the NIR region. By carefully differentiating the fluorescence signals in the ON- and OFF-state images, we envision that our reversible NIR fluorescence photoswitch may enable highly sensitive and precise imaging, particularly in the in vivo environment with background signals such as autofluorescence. |
