| 학회 |
한국고분자학회 |
| 학술대회 |
2011년 가을 (10/06 ~ 10/07, 김대중 컨벤션센터) |
| 권호 |
36권 2호 |
| 발표분야 |
의료용 고분자 부문위원회 |
| 제목 |
Polyethyleneimine-Mediated Gene Delivery into rat mesenchymal stem cells |
| 초록 |
Recently, a number of new techniques have been developed to deliver DNA into target cells. Among the cationic polymer-based formulations tested for non-viral gene transfections, polyethyleneimine (PEI) is considered one of the most efficient polymers for gene delivery. In this study, we examined the use of PEI as a non-viral gene carrier for rat bone marrow stem cells (rBMSCs) and rat muscle derived stem cells (rMDSCs). The formation of PEI-DNA complexes was characterized by gel electrophoresis and measurement of particle size, surface charge and loss of fluorescence. As the proportion of PEI was increased, the size of the PEI-DNA complexes decreased from 470 to 150 nm, the positive surface charge increased from -25 to 25 mV, and the fluorescence intensity decreased from 45 to 10. The transfection efficiency of rBMSCs was highest at N/P charge ratio 4 (16%) and that of rMDSCs was highest at N/P charge ratio 16 (5%). |
| 저자 |
권진선, 김다연, 강꽃님, 민병현, 김재호, 김문석
|
| 소속 |
아주대 |
| 키워드 |
Gene delivery; Transfection; Stem cell
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| E-Mail |
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