| 초록 |
Brown macroalgae has been considered as renewable biomass for the production of biofuels and biochemical. Major sugar components of brown macroalgae are alginate, mannitol, laminarin and fucoidan. The sugars from laminarin and mannitol can be easily used for bio–fermentation. However, alginate cannot be used directly because industrial microbes are not able to metabolize the alginate. Alginate composes 30–50% of the sugars in brown macroalgae, so disability of industrial microbes to catabolize alginate results in poor product yield. In order to use alginate together with other sugars, the recombinant oligoalginate lyase was produced by using E.coli. Alginate was successfully degraded into unsaturated uronate monosaccharides by the oligoalginate lyase lysate. In order to produce biopolymer, the recombinant E.coli was engineered to harbor the relevant genes involved in the alginate catabolism and biopolymer synthetic genes. An engineered E. coli could accumulate biopolymer. |
