| 학회 |
한국공업화학회 |
| 학술대회 |
2017년 봄 (05/10 ~ 05/12, 광주 김대중컨벤센센터(Kimdaejung Convention Center)) |
| 권호 |
21권 1호 |
| 발표분야 |
생물공학 |
| 제목 |
Optimal pathway construction to produce 3-Hydroxypropionic acid from glycerol in Escherichia coli |
| 초록 |
3-Hydroxypropionic acid (3-HP), which can be converted into many chemicals such as acrylic acid, acrylamide, is an important platform chemical. It can be biologically produced from glycerol by two enzymatic reactions. The pathway has been proved efficient, but imbalance between the rates of the two enzymatic reactions often results in the accumulation of the toxic 3-HPA, which severely reduces 3-HP production. In this study, we used UTR engineering to maximally increase the activities of two enzymes (GDHt and ALDH) for the high conversion of glycerol to 3-HP. Thereafter, the activity of GDHt was precisely controlled to avoid the accumulation of 3-HPA by varying expression level. The optimally balanced E. coli HGL_DBK4 showed a substantially enhanced 3-HP titer and productivity compared with the parental strain. Our study demonstrates that metabolic balancing through UTR engineering is an effective strategy for the development of efficient microorganisms for bio-refinery production. |
| 저자 |
이지훈1, 임현규1, 노명현1, 박성훈2, 정규열1
|
| 소속 |
1포항공과대, 2UNIST |
| 키워드 |
3-Hydroxypropionic acid; UTR designer; Metabolic engineering; Synthetic Biology
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| E-Mail |
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