| 학회 |
한국공업화학회 |
| 학술대회 |
2018년 봄 (05/02 ~ 05/04, 대구 엑스코(EXCO)) |
| 권호 |
22권 1호 |
| 발표분야 |
생물공학_포스터 |
| 제목 |
Production of 3-hydroxypropionic acid by optimal pathway rebalancing from glycerol in Escherichia coli |
| 초록 |
3-Hydroxypropionic acid (3-HP), which can be converted into many chemicals such as acrylic acid, is an important platform chemical. It can be biologically produced from glycerol by two enzymatic reactions, dehydration of glycerol to 3-hydroxypropionaldehyde (3-HPA) and oxidation of 3-HPA. The pathway has been proved efficient, but imbalance between the rates of the two enzymatic reactions often results in the accumulation of the toxic 3-HPA. In this study, we used UTR engineering to maximally increase the activities of glycerol dehydratase (GDHt) and aldehyde dehydrogenase (ALDH) for the high conversion of glycerol to 3-HP. Thereafter, the activity of GDHt was precisely controlled to avoid the accumulation of 3-HPA by varying expression of a gene encoding a main subunit of GDHt. The optimally balanced E. coli HGL_DBK4 showed a substantially enhanced 3-HP titer (40.51 g/L) and productivity (1.35 g/L/h) compared with the parental strain. |
| 저자 |
이지훈1, 임현규1, 노명현1, 박성훈2, 정규열1
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| 소속 |
1포항공과대, 2UNIST |
| 키워드 |
Metabolic engineering; Synthetic biology; Escherichia coli; 3-Hydroxypropionic acid
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| E-Mail |
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